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. 2019 May 30;20(11):2663. doi: 10.3390/ijms20112663

Figure 5.

Figure 5

Treatment with DCPIB or siRNA-mediated knockdown of LRRC8A have no effects on PI3K/Akt signaling. (A) Western blot of GBM cells treated with DCPIB (100 μM) at day 1 or day 2 for total amount and phosphorylated forms of Akt and ULK. GAPDH used as loading control. A representative blot of three independent experiments is shown. Analysis of the p-Akt/t-Akt (B,D) and p-ULK/t-ULK (C,E) ratio for U251 (B,C) and U87 (D,E) cells. (F) Western blotting of GBM cells for total amount and phosphorylated forms of Akt and ULK 72 h after siRNA transfection. For LRRC8A, the same samples were used as in Figure 4A and 4B. GAPDH used as loading control. A representative blot of three independent experiments is shown. Analysis of the p-Akt/t-Akt (G,I) and p-ULK/t-ULK (H,J) ratio for U251 (G,H) and U87 (I,J) cells. All data are presented as mean ± SD of n = 3 independent experiments.