Figure 1.

PK-resistant regions of Sup35 prions. A superposition of Sup35 peptides identified after PK digestion of Sup35NMG preparations from all [PSI⁺] isolates. The peptides were divided into six groups according to their start and end positions. Graph shows the number of identified peptides in each group, which include a given amino acid residue. In other words, the graph was obtained by placing identified peptides in accordance with their coordinates one above the other with a height of 1. Peptides of the groups 2S and 2W were found more often in Cores 2 of strong and weak [PSI⁺] variants, respectively. Peptides of the 2–3 group of fragments were observed in variants S-L20 and S-T9 at low abundance. Note that the large variation in the abundance and the frequency of appearance of the peptides is not reflected here.