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. 2019 May 29;20(11):2633. doi: 10.3390/ijms20112633

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Manipulations with Core 2. Left: the schemes of experiments. Straight lines show amyloid Cores 1 and 2; wavy line: presumably unstructured regions; pink line: two added oligopeptide repeats (9+10 residues). Right: Sup35NM PK resistance profiles before (blue line) and after experiments. (A) Attempted Core 2 elimination by overproduction of Sup35(Δ84–112)NMG with deletion covering a larger part of Core 2. (B) Core 2 was propagated in variants W-1112 and S-W8 by Sup35N(+2R)MG with 2 repeats inserted between Cores 1 and 2. The region of two added repeats was protease-sensitive and it was omitted from the profile of Sup35N(+2R)M to allow comparison of the profiles; the place of insert is indicated as “Ins”.