Table 3.
Parameters of electroporation. Depending on the electroporated tissue, the volume and concentration both hyaluronidase and plasmids suspension, as well as the number of applied pulses, pulse width, applied voltage and the type of electrode will differ.
| Muscle | ||||
|---|---|---|---|---|
| Gastrocnemious | Tibialis | Soleus | Foot Muscle: (Flexor Digitorum Brevis (FDB) and Interosseus (IO) Muscle) |
|
| Hyaluronidase in sterile Tyrode | 0.4 U/μL (30 μL) | 8 U/20 μL | - | 6 U/10 μL |
| Plasmid volume (μL) | 50 (4 × 12.5) (1–2 μg/1 μL) |
30 (2 μg/1 μL) | 20 (2 μg/1 μL) | 2–5 μg plasmid/μL volume (10–20 μL) |
| Syringe needle | 29 G | 26 G | 34 G | 33 G |
| Pulses | 2 × 4 | 5 | 8 | 20 |
| Voltage (V/cm) | 175 | 120 | 200 | 100 |
| Pulse width (ms) | 100 | 20 | 20 | 20 |
| Frequency (Hz) | 1 | 1 | 1 | 1 |
| Type of electrodes | plate electrodes 10 × 10 mm | plate electrodes 7 × 7 mm | plate electrodes 10 × 10 mm | plated acupuncture needle |