Figure 4.

Survival kinetics of rotifer populations treated (100 µM) with stimulator, toxin or calorie restriction mimetic. The number and survival of individuals significantly increased under spermidine treatment, compared to untreated controls. The cyclic adenosine monophosphate (cAMP) had a positive effect on the reproduction of individuals; however, it had no pronounced influence on the survival of animals. NaN₃ was toxic to both Philodina acuticornis (A) and Adineta vaga (B) species. The two different rotifer genera show similar kinetics under the same treatments. The error bars represent SEM. One-way ANOVA with Bonferroni post hoc test was used for statistical analysis, the level of significance is ***; ### p < 0.001 (*, significant difference in number of rotifers from untreated controls on day nine; # significant difference from untreated controls in survival of animals; n = 12 wells).