Figure 5.

Biological characteristics of rotifers treated (100 µM) with stimulator, toxin or calorie restriction mimetic having contradictory profile. On day six, three experimental- (NRA, BSI, MCF) and one chemical (CRC) parameter of individual rotifers and populations were monitored. All parameters show significant differences from their untreated controls (shown as zero value on the x axis in graphs) in a positive direction evoked by cAMP (A) in contrast to NaN₃ (B). Spermidine (C) has an antagonistic profile related to the NRA-BSI compared to CRC-MCF pairs. These three types of treatments show different biological profiles (stimulation, toxicity and antagonism) in our rotifer models. The error bars represent SEM. One-way ANOVA with Bonferroni post hoc test was used for statistical analysis, the levels of significance are * p ≤ 0.05, ** p ≤ 0.01 and *** p ≤ 0.001. (*, significant difference from untreated controls, where the corresponding data of 100% is the zero value on the x axis); NRA, n = 24 wells; BSI, n = 50 randomly selected individuals from 24 wells; CRC, n = 24 wells; MCF, n = 50 randomly selected individuals from 24 wells).