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. 2019 May 31;20(11):2678. doi: 10.3390/ijms20112678

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The expression of DRL1 during leaf senescence in grapevine. (a) Leaf senescence phenotypes in grapevine cultivar ‘Yatomi Rosa’. YL, expanding young leaf; ML, mature leaf that is fully expanded but with no visible yellowing; ES, early senescence leaf, with about 5% of the leaf area yellowing; LS, late senescence leaf, with more than 50% of the leaf area yellowing. (b) Relative expression of DRL1 during leaf senescence. The expression levels were determined using qPCR. The expression levels in LS leaves were set to 1. Bars represent mean ± standard deviation (SD). (c, d) GUS staining in leaves of P_DRL1-GUS transgenic tobacco plants at 10 d (c) and 20 d (d).