Figure 1.

The scheme of the experiment and adaptation of pluripotent cell lines in serum- and feeder-free conditions. (A) Pluripotent stem cells (colony of cells, magnification 100×) undergo formation of EBs for five days in suspension culture. The formed EBs were then transferred onto Matrigel™ coated plates for the standard 3-week differentiation in serum-free conditions with supplementation of TGFβ3. The prolonged time of differentiation was used to establish an influence on chondrogenesis. Additionally, 3D culture was performed to compare its effect on differentiation and maturation of chondrogenic like-cells. (B) The morphology of pluripotent stem cells adapted to serum- and feeder-free conditions shows that the colonies formed from compacted cells with a high rate of the nucleus to cytoplasm ratio. The white scale bar represents 50 µm. (C) The expression of pluripotency markers in adapted pluripotent cell lines indicates their increased expression in comparison with PHDF.