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Acetic acid-assisted precipitation of host cell proteins. Lane M is the protein molecular weight marker; 50% acetic acid solution was added dropwise into the crude cell extract. The supernatant treated with different pH values were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and gray scanning. After precipitation, the supernatant was readjusted to pH 9 (Lanes 4–9). The lysate, which directly adjusted from pH 7 to pH 9, was set as a control (Lanes 7–9).
