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. 2019 Jun 5;20(11):2764. doi: 10.3390/ijms20112764

Figure 4.

Figure 4

CSPG4-CAR T cells antigen-specifically kill KOPN8 leukemia cells. The different T cell conditions were generated as mentioned above (Figure 2). Mock (no RNA)-electroporated T cells and CEA-CAR T cells served as negative controls. Cytotoxicity towards T2.A1 cells, KOPN-8 cells, and A375M cells was determined 24 h after electroporation. (a) Upon 4 h of co-culture with the target cells, degranulation was assayed using CD107a and CD8 staining. Percent degranulation of CD8+ T cells was calculated by dividing the portion of CD107a-positive/CD8-positive T cells by the portion of CD8-positive T cells. Data represent means ± SEM from three independent experiments; p values were calculated by paired t test, * indicates p ≤ 0.05, and ** indicates p ≤ 0.01. (b,c). Lytic capacity towards T2.A1 (b) and KOPN8 (c) cells was analyzed at the indicated effector-to-target ratios in a standard 4 h chromium lysis assay. Data represent means ± SEM from three independent experiments; p values were calculated by paired t test, * indicates p ≤ 0.05, ** indicates p ≤ 0.01, and *** indicates p ≤ 0.001.