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. 2019 May 22;38(13):e101174. doi: 10.15252/embj.2018101174

Figure EV1. HEK293 cells transfected with K369I‐hTau‐EGFP have significantly decreased protein synthesis compared with EGFP or hTau‐EGFP expressing cells.

Figure EV1

After treatment with AHA for 4 h, HEK293 cells transfected with K369I‐hTau (1N4R)‐EGFP showed a significantly lower FUNCAT signal normalised to the EGFP signal than cells transfected with hTau (1N4R)‐EGFP or the EGFP vector alone, suggesting that protein synthesis is decreased in the mutant cells (one‐way ANOVA, Tukey's multiple comparison test, n = 3 replicates). Cells with K369I‐hTau‐EGFP and low FUNCAT signal are indicated by white filled arrows.

Data information: Mean ± SEM. **P ≤ 0.01, ***P ≤ 0.001. Scale bar: 40 μm.