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. 2019 May 27;38(13):e100532. doi: 10.15252/embj.2018100532

Figure 1. RASSF1A suppresses metastasis in lung adenocarcinoma.

Figure 1

  1. Kaplan–Meier curves for overall survival (OS) in lung adenocarcinoma TCGA_LUAD (RASSF1 mRNA high/low cutoff FKPM 5.85) and squamous cell carcinoma patients TCGA_LUSC (RASSF1 mRNA high/low cutoff FKPM 6.52). Significance derived from log‐rank test.
  2. Western blot with indicated antibodies of isogenic H1299 cells stably transfected with either empty vector pcDNA3 (H1299control) or RASSF1A (H1299RASSF1A). Bottom: cell proliferation resazurin assay. (n = 2). Error bars represent mean ± SEM.
  3. Cartoon of lung adenocarcinoma orthotopic injection, with sites of primary and metastatic tumours indicated (ipsil = ipsilateral, same lung as primary tumour; contra = contralateral in the opposite lung). Mice were euthanized to collect the lungs at day 17 or day 30 after inoculation with tumours cells into the left lung.
  4. Size of lung primary tumours measured at day 30 after lung orthotopic injection either with H1299control (n = 14 mice per group; experimental groups of 6 and 8 different shading within the groups represents two independent experiments) or with H1299RASSF1A cells stably re‐expressing RASSF1A (n = 12 mice per group; experimental groups of 6 and 6). The size of primary tumours was measured by MRI software ITK‐SNAP. Statistical significance via 2‐tailed Student's t‐test. Error bars represent mean ± SEM.
  5. Visual assessment quantification of metastatic events on the ipsilateral (left) and or contralateral (right) lungs, generated by H1299control or H1299RASSF1A at day 30. Result was calculated from two independent in vivo experiments (as in D). Graph shows significant decreasing of metastases when lungs were injected with H1299RASSF1A. Statistical significance via 2‐tailed Student's t‐test. Error bars represent mean ± SEM.

Source data are available online for this figure.