Skip to main content
. 2019 May 27;38(13):e100532. doi: 10.15252/embj.2018100532

Figure EV1. RASSF1A suppresses formation of primary tumours and metastatic progression in lung adenocarcinoma.

Figure EV1

  1. Representative longitudinal MR images of a lung tumours in an individual mouse at day 30 formed by H1299control or H1299RASSF1A overexpressed cells. Red arrowheads indicate primary tumours in ipsilateral (left) lungs. Bottom panel: Representative images of macroscopic appearances of tumour nodules on the lung surface at day 30, identified as patchy and whitish areas.
  2. Representative images of the lung primary tumours generated at day 17 after orthotopic lung injection with either H1299control or H1299RASSF1A cells, and arrows indicate lung primary tumours in ipsilateral (left) lungs (n = 4 mice per group).
  3. Representative fluorescence images of H1299control and H1299RASSF1A primary lung tumours stained for macrophages (green) with F4/80 and DAPI (blue). Images are presented as merge F4/80 with DAPI. Scale bars: 100 μm.
  4. Western blot analyses of pYAP1 protein expression in HOP92 cell lines after stable knockdown of RASSF1A by shRNA lentiviral transfection. Right: Resazurin assay used for analysing proliferation ratio in HOP92shcontrol and RASSF1A knockdown HOP92shRASSF1A cells shows great increased in proliferation when expression of RASSF1A protein was impaired. Statistical analyses were performed using Student's t‐test 2‐(tailed) of n = 2 experiments, and error bars represent the mean ± SEM.
  5. Left: Graph showing size of primary tumours formed by HOP92 lung adenocarcinoma cells after lung orthotopic injection, day 30. Knockdown of RASSF1A in HOP92 cells (shRASSF1A) (n = 7 mice per group) demonstrated higher ability to bear primary tumours that control group, when RASSF1A was expressed (shcontrol) (n = 6 mice per group). Right: Representative longitudinal MR images of a lung tumours in an individual mouse at day 30 generated by HOP92shcontrol (n = 6 mice per group) or HOP92shRASSF1A (n = 7 mice per group) by orthotopic lung injection.

Source data are available online for this figure.