Skip to main content
. 2019 Apr 26;38(13):e101153. doi: 10.15252/embj.2018101153

Figure 5. Stabilization of supplementary interactions with increasing 3′ miRNA length.

Figure 5

  1. Top: Schematic of miR‐122 isomiRs paired with a target complementary to the seed region only. Bottom: Release of the seed‐paired target from Ago2‐miR complexes was assessed by monitoring the fraction of the total target RNA bound as a function of time.
  2. Top: Schematic of miR‐122 isomiRs paired with a target complementary to the seed and supplementary (g13–g17) regions. Bottom: Fraction target bound to the Ago2‐miR complexes as a function of time.
  3. Top: Schematic of miR‐122 isomiRs paired with a target complementary to the seed and shifted supplementary (g15–g19) regions. Bottom: Fraction target bound to the Ago2‐miR complexes as a function of time.
  4. Structure of the miRNA 3′ tail suggests tension in the miRNA may restrict PAZ domain movements and opening of the supplementary chamber (target RNA not shown for clarity).
  5. Release half‐times (t 1/2) of target RNAs in panels (A–C) plotted against isomiR length.
Data information: All plotted data are the means of at least three independent replicates. Error bars indicate SEM.