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. 2019 Jun 3;38(13):e100926. doi: 10.15252/embj.2018100926

Figure 3. Toxoplasma‐infected human macrophages die through AIM2‐dependent atypical apoptosis.

Figure 3

  1. AnnV‐Glo assay from IFNγ‐primed THP‐1 transfected with siRNA against the indicated human NLR/ALR sensors and infected with type I or type II Toxoplasma gondii (Tg). Area under the curve (AUC) from real‐time assays is plotted as mean ± SEM from n = 3 independent experiments. ****P ≤ 0.0001 from two‐way ANOVA for indicated comparisons following adjustment for multiple comparisons; ns, not significant.
  2. AnnV‐Glo assay from IFNγ‐primed primary human MDMs transfected with siRNA against AIM2 or non‐targeting control (CTRL) and infected with type I or type II Tg. Area under the curve (AUC) from real‐time assays is plotted from n = 4 independent experiments. Matched shapes and color of symbols indicate donors. ****P ≤ 0.0001 from two‐way ANOVA for indicated comparisons following adjustment for multiple comparisons; ns, not significant.
  3. Representative images (top) from IFNγ‐primed THP‐1 WT infected with type I Tg for 8 h and stained for ASC and active caspase‐8. Blue, Nuclei; Red, ASC; Green, active caspase‐8 (IETD‐FITC); and Gray, Tg. Scale bar, 5 μm or 2 μm in the magnified images. Quantification of ASC specks (bottom) in Tg‐infected THP‐1 WT or ∆CASP1 treated with IFNγ or left untreated at 8 h post‐infection, plotted as mean ± SEM from n = 3 independent experiment.
  4. Immunoblots from primary MDMs infected with Tg strains for the indicated times. Cells were untreated or pre‐treated with IFNγ before infection. Images are representative of n = 4 biologically independent experiments.

Source data are available online for this figure.