Figure EV5. Transfection of pathogen‐derived ligands bypasses the requirement for GBP1 in cell death.

• A, B
  Annexin V (AnnV)‐Glo assay kinetic of primary monocyte‐derived macrophages (MDM) transfected with non‐targeting control (CTRL) or GBP1 siRNA (A) and THP‐1 or ΔGBP1 cells (B) and transfected with soluble Toxoplasma gondii antigen (STAg). Cells were unprimed or primed with IFNγ before experiments. Mean ± SEM from area under the curve (AUC) of 18‐h real‐time assays plotted from n = 4 independent experiments.
• C
  Propidium iodide (PI) uptake assay from indicated THP‐1 cells left untreated (UT), treated with LPS or transfection reagent (Lipofectamine) only, or transfected with LPS (for 4 h) with IFNγ‐priming. Area under the curve (AUC) from real‐time assay plotted as mean ± SEM from n = 3 independent experiments.
• D
  IL‐1β ELISA from THP‐1 cells transfected with non‐targeting control (CTRL) or GBP1‐5 siRNA as indicated which were left untreated or primed with IFNγ and transfected with LPS. Mean ± SEM from area under the curve (AUC) of 24‐h real‐time assays plotted from n = 3 independent experiments.

Data information: ****P ≤ 0.0001 from two‐way ANOVA following adjustment for multiple comparisons; ns, not significant.