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. 2019 May 29;20(11):2640. doi: 10.3390/ijms20112640

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cav-1 and Cav-3 mRNA levels. Histogram shows the results of the real-time PCR experiments of mice whole-heart tissue. Upper panel is Cav-1 over GAPDH. and lower panel is Cav-3 over GAPDH (n = 5 for control and n = 6 for helium). No significant changes were observed, suggesting that the increase of Cav-3 in exosomes from PFP at this time point is not due to higher levels of Cav-3 gene expression but through a different mechanism, such as the direct release of Cav-3 from plasma membranes.