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. 2019 Jun 6;24(11):2138. doi: 10.3390/molecules24112138

Figure 2.

Figure 2

Oral administration of EGCG suppresses acute gout symptoms induced by MSU crystal injection in mice. Mice were orally administered EGCG (1, 10, and 30 mg/kg) or vehicle (0.02% dimethyl sulfoxide (DMSO) in water). After 1 h, MSU crystals (2 mg/0.1 mL of phosphate-buffered saline (PBS)/mouse) or PBS was subcutaneously injected into the footpad of the right hindfoot of each mouse. After 24 h, the footpad tissues were collected for further analysis. (A) Time course of footpad thickness gain compared with the footpad thickness at the 0 h time point per group. (B) Representative picture of hematoxylin and eosin staining of the hind feet. Infiltrating neutrophils in the hindfoot tissue appear as purple dots. (C) Representative pictures of immunohistochemistry staining of foot tissues with myeloperoxidase (MPO) (200×). (D) Supernatants of the foot tissue homogenates were analyzed for IL-6 by ELISA. The values in the line and bar graphs represent the means ± SD (n = 6 mice/group). #, significantly different from vehicle group, p < 0.05; *, significantly different from MSU alone, p < 0.05.