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Working memory is impaired in mice fed with amino acid imbalanced food. (a) PS1 and ATF4 expression are significantly increased in the brain of mice fed with leucine- and lysine-deficient food (LLD) 30 minutes per week, for ten times. (b) Percentage of alternation measured in the Y-maze test decreased in mice fed an LLD. The protocol of animal study was approved by the Gifu University Graduate School of Medicine Animal Care and Use Committee of 20-131 (15/1/2009).
