Figure 7.

Adult CPCs treated with 17-AAG demonstrate upregulated YAP1 and SOD2 expression. Adult CPCs were treated with four concentrations of drug over three different time points for treatment optimization (A). 1 uM of drug produced the most consistent results with YAP1 expression increasing after 72 h (B). SOD2 expression was elevated in a similar pattern with higher fold changes (C). Gel electrophoresis was used to confirm the size of the SOD2 transcript (D). From left: Ladder, non-treated control, 24, 48, and 72 h treatment of 1uM 17-AAG. * p < 0.05, ** p < 0.01, *** p < 0.001. Fold changes are shown as the mean ± SEM. All samples were run in triplicate.