Figure 2.

Gal-9 induces apoptosis of ESCC cells. (A) Early apoptosis analysis in KYSE-150 cells 6 h after the administration of Gal-9. The lower left quadrant represents viable cells (red dots), the lower right quadrant early apoptotic cells (green dots), and the upper right quadrant indicates the late apoptotic or necrotic cells (blue dots); (B) The transition of Annexin V-positive cell number following treatment with Gal-9 for 1, 6, 12, and 48 h. Annexin V-positive cells were significantly increased at 1 h and 6 h after Gal-9 treatment, compared to the control; (C) The activation of caspase-3 was observed after 6 h of treatment with 100 nM Gal-9, as evaluated with the colorimetric assay. No significant change in the activity of caspase-4, -7, -8 and -9 was observed. The error bars represent 95% CI.