Figure 4.

Gal-9 induces the collapse of the mitochondrial potential. KYSE-150 cells were treated with 100 nM Gal-9 for 3 h and then treated with JC-1 dye for 15 min. Cells were then visualized by a fluorescent microscope to examine the conversion of red to green color in healthy and apoptotic cells, respectively. Healthy cells were stained red with JC-1 dye using a filter set excitation/emission 540/570 nm. Apoptotic cells were stained green with JC-1 dye using a filter set excitation/emission 485/535 nm, suggesting that a collapse of mitochondrial potential by Gal-9 treatment. Scale bars are shown for the images.