Table 1.

International Organization of Standardization (ISO) 10993-5:2009 criteria for reactivity scoring of the agar diffusion assay [47].

Grade	Reactivity	Reactivity Zone Description
0	None	No detectable zone around or under the specimen
1	Slight	Some malformed or degenerated cells under the specimen
2	Mild	Zone limited to the area under the specimen
3	Moderate	Zone extending the specimen size up to 1.0 cm
4	Severe	Zone extending farther than 1.0 cm beyond the specimen