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SilA thermal inactivation after incubation at 95 °C. The inset represents the first-order kinetics of deactivation (ln activity = ln activity(t = 0) – kdt, where kd is the rate constant of deactivation). The calculated half-life of inactivation (t1/2 = ln 2/kd), of SilA at 95 °C was 1.3 h.
