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LC separations of the ginsenoside mixture on the ACE 3 C18 column using different column temperatures and the mobile phase gradient shown in Table 1. The mobile phase flow rate and initial composition was 0.7 mL/min and 22 % ACN, respectively. Peak identification is the following: (1) Rg1, (2) Re, (3) Rf, (4) Rb1, (5) Rc, (6) Rg2, (7) Rh1, (8) Rb2, (9) Rb3, and (10) Rd.
