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. Author manuscript; available in PMC: 2019 Nov 2.
Published in final edited form as: J Chromatogr A. 2018 Sep 7;1574:114–121. doi: 10.1016/j.chroma.2018.09.011

Table 1.

Mobile phase gradient for the LC/UV and LC/MS methods.

LC/UV
 LC/MS
Time H2O (%) ACN (%) Time H2O/0.1% FA (%) ACN (%)
−5.0 78 22 0.0 78 22
0.0 78 22 10.0 78 22
10.0 78 22 38.0 42 58
29.0 58 42 45.0 0 100
59.0 0 100 60.0 0 100
60.0 0 100 65.0 78 22
60.1 78 22 70.0 78 22