Table 1.
Phenotypical traits utilized to characterize the suspected colonies grown on CHROMagar™ Vibrio: V. alginolyticus colorless, V. parahaemolyticus mauve, V. cholerae/V. vulnificus green blue to turquoise blue. Expected results.
| TCBS Agar | m-CPC Agar | Gram-roads | Oxidase | Nitrate reduction | O129/150 μg S/R O129/10 μg S/R | SIM motility/indole/H2S | A/L/O | KIA | |
|---|---|---|---|---|---|---|---|---|---|
| VA | Y | ng | + | + | + | SR | +/+/- | -/+/+ or - | GF+/L-/no H2S/no gas |
| VC | Y | P/G | + | + | + | SS | +/+/- | -/+/+ or - | GF+/L-/no H2S/no gas |
| VV | G/Y | Y/W/ng | + | + | + | SS | +/+/- | -/+/+ or - | GF+/L+ or L-/no H2S/no gas |
| VP | G | ng | + | + | + | SR | +/+/- | -/+/+ or - | GF+/L-/no H2S/no gas |
VA, V. alginolyticus; VP, V. parahaemolyticus; VC/VV, V. cholerae/V. vulnificus; Y, yellow; G, green; W, white; P, purple; ng, no growth; GF, glucose fermentation; L, lactose utilization; SIM, Sulfide Indole Motility medium; A, arginine dihydrolase; L, lysine decarboxylase; O, ornithine decarboxylase; KIA, Kligler Iron Agar.