Table 1.
Antibodies used for immunofluorescence and for flow cytometry analysis
| Antigen | Company | Dilution | Incubation time | |
| Immunofluorescence staining | ||||
| CD73 | Thermo Fisher | Weston, Florida, USA | 1:100 | 1 h |
| CD90 | Abcam | Cambridge, UK | 1:100 | 1 h |
| CD105 | Dako | Palo Alto, California, USA | 1:10 | 1 h |
| CD34 | 1:100 | 30 min | ||
| CD45 | 1:100 | 30 min | ||
| CD56 | 1:80 | 30 min | ||
| CD146 | Santa Cruz | Dallas, Tx, USA | 1:25 | 1 h |
| PDGFRα | 1:25 | 1 h | ||
| PW1 | Atlas Antibodies | Bromma, Sweden | 1:100 | 1 h |
| Alexa Fluor | 1:700 | 40 min | ||
| 488 nm | ||||
| Donkey anti-mouse | ||||
| Alexa Fluor | Thermo Fisher | Weston, Florida, USA | 1:700 | 40 min |
| 488 nm | ||||
| Donkey anti-rabbit | ||||
| Alexa Fluor | 1:700 | 40 min | ||
| 594 nm | ||||
| Donkey anti-mouse | ||||
| Alexa Fluor | 1:700 | 40 min | ||
| 594 nm | ||||
| Donkey anti-rabbit | ||||
| Flow Cytometry | ||||
| CD73 (IgG) APC | BD Pharmingen | San Jose, California, USA | 1:25 | 30 min |
| CD90 (IgG) FITC | 1:25 | 30 min | ||
| CD105 (IgG) PE | 1:25 | 30 min | ||
| CD34 (IgG) FITC | 1:25 | 30 min | ||
| CD45 (IgG) APC | 1:25 | 30 min | ||
| CD56 (IgG) PE | 1:25 | 30 min | ||
| CD146 (IgG) PE | 1:25 | 30 min | ||
| Isotype controls: | 1:25 | 30 min | ||
| IgG APC | ||||
| IgG PE | ||||
| IgG FITC | ||||