Figure 3. Kisspeptin projections to the median eminence intermingle intimately with GnRH fibers in the monkey.

Interactions between kisspeptin (green fluorescence) and GnRH (red fluorescence) fibers in hemi-coronal sections at the anterior ( A), mid-tuberal ( B), and posterior ( C) aspects of the median eminence of a castrated adult male rhesus monkey. Note, in panel B, the heavy kisspeptin innervation of the internal zone of the median eminence and GnRH innervation of both the internal and external zones. Panel D shows high-power magnification of kisspeptin axonal beads in contact with GnRH fibers (white arrows) in the external zone of the median eminence shown in the inset. GnRH, gonadotropin-releasing hormone. Reproduced from Ramaswamy et al. ⁴⁴ with permission of the Endocrine Society.