Fig. 7.

Transendothelial migration of hES-MSCs. BMECs were plated on 24-well Transwell inserts, allowed to achieve confluence, and then PKH-26 labeled hES-MSCs or BM-MSCs were added to top of chamber. a After 6 h and 24 h at 37 °C, migrated hES-MSCs and BM-MSCs were collected from the from bottom chamber and counted. b Epifluorescence images of hES-MSCs and BM-MSCs that migrated into the bottom chamber at 6 h and 24 h. Data are presented as mean ± SE. Each experiment consisted of 3 replicates (derived from a single preparation of BMECs) repeated 3 times (each time from a different BMEC preparation), for a total N = 9 samples per group. *p < 0.05 compared with BM-MSC group