Table 2.
Summary of methods’ application to image data
| Manual count | (S1) | (S2) | (S3) | (S4) | (S5) | (S6) | (GE) | |
|---|---|---|---|---|---|---|---|---|
| ROIs | ||||||||
| # single-channel images at CD14 +/488 nm analyzed | 25 | 25 | 25 | 25 | 25 | 17 | 17 | − |
| # single-channel images at CD163 +/555 nm analyzed | 25 | 25 | 25 | 25 | 25 | 18 | 18 | − |
| DAPI channel used | no | no | no | no | no | no | no | − |
| Whole tissue samples | ||||||||
| # single-channel datasets at CD14 +/488 nm analyzed | − | 44 | 44 | 43 | 41 | − | − | 37 |
| # single-channel datasets at CD163 +/555 nm analyzed | − | 44 | 44 | 43 | 41 | − | − | 37 |
| DAPI channel used | − | yes | yes | yes | yes | − | − | no |
| Tumor subregions | ||||||||
| # single-channel datasets at CD14 +/488 nm analyzed | − | 44 | − | − | − | − | − | − |
| # single-channel datasets at CD163 +/555 nm analyzed | − | 44 | − | − | − | − | − | − |
| DAPI channel used | − | yes | − | − | − | − | − | − |