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. 2019 Jun 10;116(26):12974–12979. doi: 10.1073/pnas.1902844116

Fig. 1.

Fig. 1.

AMPK phosphorylation of NCL. (A) miR binding protein ranking based on the number of putative targeted miRs. (B) Functional domains and AMPK phosphorylation consensus sequences on human, mouse, and rat NCL. (C) In vitro kinase assay demonstrating the phosphorylation of recombinant NCL by AMPK. (D) Isoelectric focusing of NCL isolated from AMPK+/+ and AMPK−/− MEF cells. (E) In vitro kinase assay using recombinant AMPK and NCL peptides (11-mers) illustrated in B. (F) Peptide competition assay against SAMS peptide and increasing concentrations of S328A 11-mer peptide. (G) Immunoblot with p-Serine antibody for FLAG-NCL immunoprecipitated from HUVEC treated with AICAR for 10 min. (H and I) Immunoblotting of lysates from HUVECs under PS and OS as well as TA and AA. *P < 0.05. Data are mean ± SEM from three independent experiments.