Fig. 1.

PolyP-induced amyloid formation of β2m at acidic pH. (A) Chemical structure of polyP. n is the degree of polymerization of the phosphate group. (B) Repeated measurements of spectra with an excitation at 445 nm in the presence of polyP-L. Inset shows an expanded image of ThT fluorescence. (C and D) Kinetics of amyloid formation monitored by ThT fluorescence at 485 nm (blue) and total aggregation monitored by LS at 442 nm (red) at various concentrations of orthoP (C) and polyP-L (D) at 37 °C under ultrasonication. β2m was added at the time indicated by the arrowhead.