Fig. 3.

Increased lung microvessel pressure induces Piezo1-dependent lung vascular hyperpermeability secondary to reduced expression of AJ proteins. (A) Effects of 50% constriction of aorta (TAC) in Piezo1^fl/fl and Piezo1^iΔEC mice on left atrial pressure (Left) and left ventricular systolic pressure (Right) recorded at 24 h post-TAC. (B and C) Accumulation of lung water (B) and increased lung transvascular albumin permeability (PS) (C) were blocked in Piezo1^iΔEC mice and in Piezo1^fl/fl mice treated with GsMTx4 at 24 h post-TAC. (D and E) Loss of VE-cadherin, β-catenin, and p120-catenin at 24 h post-TAC. Individual data points are shown as well as mean ± SEM; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.