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. 2019 Jun 10;116(26):13097–13106. doi: 10.1073/pnas.1903203116

Fig. 3.

Fig. 3.

BDNF-dependent GR-PO4 is required for training-evoked spine survival and motor skill retention. (A) Effect of BDNF-Val66Met polymorphism on GR-PO4 staining in Thy1-YFP neurons of M1 cortex after 2 d of rotarod training. Mean ± SEM of n = 5 untrained mice per group, 7V (BDNF-Val/Val), and 8M (BDNF-Met/Met) trained; two-way ANOVA (F1,21 = 7.75, P = 0.011). Pairwise group comparison between BDNF-M and BDNF-V by unpaired t test [t(13) = 2.24, *P < 0.05]. (B) Effect of genotypes on rotarod performance at recall on day 14 expressed as a percentage of day 1 in GR-KI mice backcrossed with the BDNF-Val66Met background. Mean ± SEM of n = 8 V;WT mice, 8 M;WT mice, 9 V;KI mice, and 11 M;KI mice; three-way ANOVA: effect on retention (F1,64 = 23.68, P < 0.0001), post hoc Tukey test (*P < 0.05). ns, not significant. (C) Spine formation in M1. Mean ± SEM of n = 7 V;WT trained mice, n = 7 M;WT trained mice, n = 7 V;KI trained mice, n = 8 M;KI trained mice, n = 8 V;WT untrained mice, and n = 5 M;WT untrained mice; two-way ANOVA: effect of genotype (F3,50 = 4.65) and time (F1,50 = 91.55, P < 0.01), post hoc Tukey test (*P < 0.0001). Spine deletion. Effect of genotype (F3,50 = 12.66) and time (F1,50 = 73.12, P < 0.0001), post hoc Tukey test (*P < 0.05). d, day. (D) Survival of new spines in M1 formed on day 3 and old spines formed before day 0. Mean ± SEM of n = 7 V;WT mice, n = 8 M;WT mice, n = 9 V;KI mice, and n = 11 M;KI mice; two-way ANOVA: effect of genotype (F3,62 = 2.88, P < 0.05). Pairwise comparisons by unpaired t test between V;WT mice and M;WT mice on new spines [t(13) = 2.28] and old spines [t(13) = 4.86]; V;WT mice and V;KI mice on new spines [t(14) = 2.24] and old spines [t(14) = 9.95]; and V;WT mice and M;KI mice on new spines [t(16) = 2.15] and old spines [t(16) = 7.91, *P < 0.05].