Table 3.
Transferability of vancomycin and kanamycin resistance from donors (W. cibaria) to recipients (E. faecalis and L. rhamnosus) (CFU/mL).
| Antibiotics | Donor | Recipients | Transconjugants | Transfer Frequency |
||
|---|---|---|---|---|---|---|
| CMU | Ef | Lrh | CMU + Ef | CMU + Lrh | ||
| Van 64 | 1.03 × 108 ± 8.41 × 107 |
0 | - | - | - | - |
| Tet 16 | 0 | 3.70 × 108 ± 3.68 × 108 | - | - | - | - |
| Van 64 + Tet 16 | 0 | 0 | - | 0 | - | 0 |
| Kan 64 | 2.50 × 106 ± 7.07 × 105 |
- | 0 | - | - | - |
| Fus 64 | 0 | - | 2.15 × 109 ± 7.07 × 106 |
- | - | - |
| Kan 64 + Fus 64 | 0 | - | 0 | - | 0 | 0 |
Van 64, vancomycin (64 mg/L); Kan 64, kanamycin (64 mg/L); Tet 16, tetracycline (16 mg/L); or Fus 64, fusidic acid (64 mg/L) were included in the single- or double-selective agar medium. CMU, W. cibaria CMU; Ef, E. faecalis ATCC 29212; and Lrh, L. rhamnosus GG.