. 2019 May 31;20(11):2693. doi: 10.3390/ijms20112693

Table 7.

Gene-specific primers and conditions for polymerase chain reaction (PCR) detection.

Antibiotics	Target Genes	Oligo Sequences (5′–3′)	Annealing Temperature (°C)	Amplicon Size (bp)	References
GEN	aac(6′)-aph(2”)	CCAAGAGCAATAAGGGCATA	60	220	[51]
	aac(6′)-aph(2”)	CACTATCATAACCACTACCG	60	220	[51]
	aac(6′)Ie-aph(2”)La	CAGAGCCTTGGGAAGATGAAG	58	348	[52]
	aac(6′)Ie-aph(2”)La	CCTCGTGTAATTCATGTTCTGGC	58	348	[52]
CHL	catA	GGATATGAAATTTATCCCTC	50	486	[51]
	catA	CAATCATCTACCCTATGAAT	50	486	[51]
	cat	TTAGGTTATTGGGATAAGTTA	48	300	[53]
	cat	GCATGRTAACCATCACAWAC	48	300	[53]
STR	aadA	ATCCTTCGGCGCGATTTTG	56	282	[54]
	aadA	GCAGCGCAATGACATTCTTG	56	282	[54]
	aadE	ATGGAATTATTCCCACCTGA	50	565	[54]
	aadE	TCAAAACCCCTATTAAAGCC	50	565	[54]
	ant(6)	ACTGGCTTAATCAATTTGGG	53	597	[55]
	ant(6)	GCCTTTCCGCCACCTCACCG	53	597	[55]
VAN	vanE	TGTGGTATCGGAGCTGCAG	52	513	[56]
	vanE	GTCGATTCTCGCTAATCC	52	513	[56]
	vanX	TCGCGGTAGTCCCACCATTCGTT	55	454	[57]
	vanX	AAATCATCGTTGACCTGCGTTAT	55	454	[57]
AMP	blaZ	ACTTCAACACCTGCTGCTTTC	58	240	[58]
	blaZ	TAGGTTCAGATTGGCCCTTAG	58	240	[58]
	bla	CATARTTCCGATAATASMGCC	51	297	[53]
	bla	CGTSTTTAACTAAGTATSGY	51	297	[53]
	mecA	GGGATCATAGCGTCATTATTC	58	1429	[58]
	mecA	AGTTCTGCAGTACCGGATTTGC	58	1429	[58]
TET	tet(M)	GGTGAACATCATAGACACGC	55	401	[59]
	tet(M)	CTTGTTCGAGTTCCAATGC	55	401	[59]
	tet(K)	TCGATAGGAACAGCAGTA	55	169	[58]
	tet(K)	CAGCAGATCCTACTCCTT	55	169	[58]
	tet(W)	GAGAGCCTGCTATATGCCAGC	64	168	[56]
	tet(W)	GGGCGTATCCACAATGTTAAC	64	168	[56]
RIF	rpoB	TAACCGTGGTGCTTGGCTDGAATWYGAAAC	59	1100	[60]
RIF	rpoB	ATCAAACCAATGTTAGGNCCTTCWGGDGTTTC	59	1100	[60]
KAN	aph (3”)-III	GCCGATGTGGATTGCGAAAA	52	292	[54]
	aph (3”)-III	GCTTGATCCCCAGTAAGTCA	52	292	[54]
	ant(2”)-I	GGGCGCGTCATGGAGGAGTT	67	329	[54]
	ant(2”)-I	TATCGCGACCTGAAAGCGGC	67	329	[54]
	aph(3”)-I	AACGTCTTGCTCGAGGCCGCG	68	670	[54]
	aph(3”)-I	GGCAAGATCCTGGTATCGGTCTGCG	68	670	[54]
CIP	gyrA	GAYTATGCWATGTCAGTTATTGT	45	286	[54]
	gyrA	GGAATRTTRGAYGTCATACCAAC	45	286	[54]
	parC	TATTCYAAATAYATCATTCARGA	50	286	[53]
	parC	GCYTCNGTATAACGCATMGCCG	50	286	[53]
CLIN	lnu(A)	GGTGGCTGGGGGGTAGATGTATTAACTGG	55	323	[56]
	lnu(A)	GCTTCTTTTGAAATACATGGTATTTTTCGATC	55	323	[56]
	lnu(B)	CCTACCTATTGTTTGTGGAA	54	925	[57]
	lnu(B)	ATAACGTTACTCTCCTATTTC	54	925	[57]
ERY	erm(B)	GAAAAGGTACTCAACCAAATA	54	639	[58]
	erm(B)	AGTAACGGTACTTAAATTGTTTAC	54	639	[58]
	erm(B)-1	CATTTAACGACGAAACTGGC	54	405	[55,59]
	erm(B)-1	GGAACATCTGTGGTATGGCG	54	405	[55,59]
	erm(C)	TCAAAACATAATATAGATAAA	50	642	[58]
	erm(C)	GCTAATATTGTTTAAATCGTCAAT	50	642	[58]
LIN	cfr	TGAAGTATAAAGCAGGTTGGGAGTCA	55	746	[61]
LIN	cfr	ACCATATAATTGACCACAAGCAGC	55	746	[61]
Conjugative transposon	int (Tn916/Tn1545)	GCGTGATTGTATCTCACT	55	1046	[62]
Conjugative transposon	int (Tn916/Tn1545)	GACGCTCCTGTTGCTTCT	55	1046	[62]

GEN, gentamicin; CHL, chloramphenicol; STR, streptomycin; VAN, vancomycin; AMP, ampicillin; TET, tetracycline; RIF, rifampicin; KAN, kanamycin; CIP, ciprofloxacin; CLIN, clindamycin; ERY, erythromycin; and LIN, linezolid.