Figure EV1. Skewness of the velocity distributions for unwinding and rewinding for the different protein combinations.

1. Histograms of the observed unwinding and rewinding velocities for Sgs1 in the presence of 50 nM RPA. The mean unwinding rate was 46 ± 3 bp/s (N = 597). The mean rewinding rate was 68 ± 3 bp/s (N = 594).
2. The skewness was calculated for the data shown in the velocity histograms in the main text.