Skip to main content
. 2019 Jun 7;38(13):e101516. doi: 10.15252/embj.2019101516

Figure EV3. Top3‐Rmi1 cleavage activity of ssDNA.

Figure EV3

  1. Scheme illustrating the Top3‐Rmi1 ssDNA cleavage activity observed in the magnetic tweezers assay.
  2. Stepwise DNA length increase seen in the presence of Top3‐Rmi1.
  3. Histogram of the size of the stepwise length increase having a mean of 8.6 ± 0.2 nm (N = 433).
  4. dsDNA processing events observed for Sgs1 in the presence of Top3‐Rmi1. Stepwise DNA elongation due to ssDNA cleavage by Top3‐Rmi is observed together with dsDNA unwinding–rezipping bursts by Sgs1.