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. 2019 Jun 25;11:5821–5834. doi: 10.2147/CMAR.S198615

Figure 6.

Figure 6

A series of rescue experiments were conducted to confirm that HMGB1 downregulation mediates the activities of miR-548b in hepatocellular carcinoma (HCC) cells. Hep3B cells were cotransfected with miR-548b mimics and pCMV-HMGB1 or pCMV, whereas the miR-548b inhibitor was cotransfected with si-HMGB1 or si-negative control (NC) into SK-HEP-1 cells. (A) The protein amount of HMGB1 was determined by Western blotting with GAPDH as an internal reference. *P<0.05 as compared with NC mimics. **P<0.05 as compared with miR-548b mimics+pCMV. #P<0.05 relative to the NC inhibitor. ##P<0.05 as compared with miR-548b inhibitor+si-NC. (B–F) The proliferation, colony formation, apoptosis, migration, and invasion abilities of the aforementioned cells were assessed by the MTT assay, clonogenic assay, flow cytometry, and Transwell migration and invasion assays, respectively. *P<0.05 as compared with NC mimics. **P<0.05 as compared with miR-548b mimics+pCMV. #P<0.05 in comparison with the NC inhibitor. #P<0.05 as compared with miR-548b inhibitor+si-NC.