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. 2019 Jan 10;104(7):1388–1395. doi: 10.3324/haematol.2018.206417

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Figure 1. — Frequency of side population cells in zebrafish acute lymphoblastic leukemia correlates with leukemia-propagating cell frequency. (A, B) Side population (SP) assay performed on a zebrafish acute lymphoblastic leukemia (ALL) tumor without (A) or with (B) verapamil inhibitor to block dye efflux. Live, singlet, tumor cells are shown separated by their emission of Hoechst blue (450/50 nm bandpass filter) and Hoechst red (670/30 nm bandpass filter). Data are shown for one representative tumor of 57 tested. (C) Correlation of the percentage of SP cells versus the frequency of leukemia-propagating cells (LPC).