Figure 6.

ERGdel patterns. Three different patterns of ERGdel are depicted. (Sub)clones with distinct ERGdel are represented by different colors; cells without ERGdel are shown in gray. Gain of ERGdel in leukemia-founding cell resulting in clonal pattern is probably extremely rare, if present at all. In acute lymphoblastic leukemia with a pseudoclonal pattern, only a single ERGdel gained by progeny of leukemia-founding cell is detected. However, an additional distinct subclonal ERGdel may co-exist at the level below sensitivity of the detection methods. Our data suggest that the most frequent ERGdel pattern is polyclonal, where multiple distinct ERGdel subclones co-exist, and based on the total proportion of ERGdel-positive cells, the ERGdel can be detected by single nucleotide polymorphism array/polymerase chain reaction (PCR) or by PCR only. Patterns were visualized using fishplot package for R.