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. 2019 Jan 17;104(7):1460–1472. doi: 10.3324/haematol.2018.194555

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Figure 4. — GFI1B^Q287* of induced pluripotent stem cell-derived megakaryoid cells phenocopy disease characteristics. (A) Cytospins of control and GFI1B^Q287* induced pluripotent stem cell (iPSC) lines differentiated towards megakaryocytes (MK) and stained with May-Grünwald Giemsa. Pictures were taken at 40× magnification using a Zeiss Scope.A1 microscope (Zeiss) and images were processed with Zen blue edition. (B) Representative flow-cytometric analysis of control and GFI1B^Q287* iPSC-derived cells for surface expression of megakaryocyte-associated markers CD34, CD41a and CD42b. (C-E) Normalized median fluorescence intensity (nMFI) of CD34 (C), CD42b (D) and CD41a (E). (F) Quantification of CD41a⁺ megakaryocytic cells per seeded iPSC to measure expansion potential. CD41a⁺ cells were negative for erythroid, myeloid and endothelial makers indicating that these cells represent true megakaryocytic cells (data not shown) **P<0.01, ****P<0.0001.