Figure 3.

STAT3 signaling is significantly impaired in the arcuate nucleus (ARC) and completely abrogated in the ventromedial nucleus (VMN) and dorsomedial nucleus (DMN) in the hypothalamus of LepRb^G506S/G506Smice. (A and E) Immunohistochemistry of pSTAT3 on brain slices. (B) Quantification of pSTAT3-positive neurons in the ARC and in the VMN. Data of 2 separate experiments were pooled, in one experiment db/db were replaced by LepR^loxTB/loxTB mice. (C) Co-staining of pSTAT3 and POMC on brain sections. (D) Quantification of POMC positive neurons and frequency of double positive neurons among POMC positive neurons. (F) Quantification of pSTAT3-positive neurons in the DMN. Mice were fasted for 2h prior to leptin injection and brains were removed 45 min later. Values represent mean with standard deviation (n = 3–6/group). *, P < 0.1234; **, P < 0.0332; ***, P < 0.0021, ****, P < 0.0002.