Differences in metabolic parameters of 20 weeks old db/db and LepRbG506S/G506Smice. (A–B) Distribution of body mass in subcutaneous fat, visceral fat and lean mass represented as percentage of total body weight or gram of different tissues. (C) Fasting glucose levels. (D) Fasting glucose levels normalized to body weight. (E and F) Glucose and insulin tolerance test. Stars indicate comparison to controls, squares indicate comparison between db/db and LepRbG506S/G506S mice. (G–J) Fasting plasma levels of insulin (G), leptin (H), cholesterol (I) and triglycerides (J). (A–D) and (G–J) values represent mean and standard deviation, (E and F) values represent mean and standard error. C–E: n = 7–11; A, B, G, I, J: n = 6; F: n = 4–6; H: n = 3. *, P < 0.1234; **, P < 0.0332; ***, P < 0.0021, ****, P < 0.0002.