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. 2019 Jun 14;18(2):1449–1457. doi: 10.3892/etm.2019.7676

Figure 3.

Figure 3.

Identification of IGF-1R as a direct target of miR-877 in non-small cell lung cancer cells. (A) Sequence alignment of miR-877 and the 3′-UTR of IGF-1R. The wt and mut miR-877 binding sites in the 3′-UTR of IGF-1R are presented. (B) Luciferase reporter assay in H460 and A549 cells which were co-transfected with miR-877 mimics or miR-NC and psiCHECK-IGF-1R-3′-UTR-wt or psiCHECK-IGF-1R-3′-UTR-mut. (C) Reverse transcription-quantitative PCR and (D) western blot analyses were conducted to detect the IGF-1R mRNA and protein expression in H460 and A549 cells transfected with miR-877 mimics or miR-NC. *P<0.05 vs. miR-NC. 3′-UTR, 3′-untranslated region; IGF-1R, insulin-like growth factor 1 receptor; miR-877, microRNA-877; miR-NC, microRNA mimics negative control; mut, mutant; wt, wild-type.