Table I. Detection of representative inner and outer envelope membrane proteins. Protein accessions are provided (AGI numbers) together with their short names (Name). Detection (X) of these proteins in the present work, in the work published by Simm et al. (Core proteome, see 14), or by Ferro et al. (AT_CHLORO database, see 12) is indicated. The enrichment factor (EF) of each protein in the envelope fraction compared to the crude cell extract is indicated in the EF estimator column. na, means that the protein was detected in the whole chloroplast fraction but not in the purified envelope fractions. Bold accessions are known envelope proteins that were not detected in previous studies targeting the envelope proteome.
| AGI | Name | This work (2019) | Core proteome (2014) | AT_CHLORO database (2010) | EF estimator (this work) |
|---|---|---|---|---|---|
| AT1G04940 | TIC20-I | X | – | – | 10.8 |
| AT2G47840 | TIC20-II | X | – | – | 7 |
| AT5G55710 | TIC20-V | X | – | na | 0.7 |
| AT3G23710 | TIC22-III | X | – | X | 10.9 |
| AT4G33350 | TIC22-IV | X | X | X | 12 |
| AT4G23430 | TIC32-IVa | X | – | X | 10 |
| AT5G16620 | TIC40 | X | X | X | 17.4 |
| AT2G24820 | TIC55-II | X | X | X | 8.8 |
| AT4G25650 | TIC55-IV | X | X | X | 94.3 |
| ATCG01130 | TIC214 | X | X | X | 41.8 |
| AT2G25660 | TIC236 | X | – | – | 15.9 |
| AT5G22640 | TIC100 | X | X | X | 90.2 |
| AT1G06950 | TIC110 | X | X | X | 10 |
| AT5G20300 | TOC90 | X | – | – | 8.9 |
| AT3G16620 | TOC120 | X | X | – | 5.2 |
| AT1G63900 | SP1 | X | – | – | 8.0 |
| AT1G21650 | SECA2 | X | – | – | 27.8 |
| AT2G31530 | SCY2 | X | – | X | 7.9 |
| AT1G45170 | OEP24A | X | X | – | 1.9 |
| AT3G52230 | OEP24 | X | X | X | 4.1 |
| AT5G42960 | OEP24-II | X | – | X | 1.9 |