Fig. 1.

A proteomics screen for physical interactors of SET1A-complex subunits under endogenous conditions. A, SETD1A-complex members were immunoprecipitated from nuclear extracts of HEK293T cells. Physically associated proteins were trypsinized on beads, and the resulting peptides identified and quantified using Q Exactive mass spectrometry. B, Input nuclear lysate and samples immunoprecipitated using control- (IgG), SETD1A-, RBBP5-, and ASH2L-specific antibodies were analyzed by Western blotting for reactivity using the same antibodies. C, Spectral counts (the frequency of identification quality MS2 events per 60min LCMS run) are plotted for each immunoprecipitation experiment (carried out in triplicate).