Fig. 8.

LOX/LOXL2 inhibition abrogates extracellular matrix orientation in prostate CAF. A, Representative images of fibronectin staining show ECM fiber alignment for cell-derived matrices produced by NPF and CAF or CAF treated with the LOX/LOXL2 inhibitor DPEN or selective LOXL2 inhibitor PXS-S2A. Images were processed and color-coded to represent the degree of fiber orientation distribution within each sample; scale bar = 50 μm. Quantification of fiber alignment for NPF and CAF treated with B, DPEN or (C) PXS-S2A from Patient 4 and 6. Lines represent mean values (n = 3). *p value <0.05 compared with CAF. Raw images are shown in supplemental Fig. S7.