Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Apr 11;18(7):1454–1467. doi: 10.1074/mcp.TIR119.001431

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Amon et al.

Published by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

PMC Copyright notice

Fig. 3. — Proteome profiles of human hematopoietic stem and progenitor cell subpopulations. (A) Human hematopoietic cell hierarchy with respective cell surface markers depicted in blue (15–17). (B) FACS strategy, depicted on magnetic-activated cell sorting-preselected CD34+ hematopoietic cells isolated from healthy HSPC donors. Shown are the analysis gates. Highly enriched HSCs/MPPs (referred to as HSCs) are CD34+CD38-CD45RA-, highly enriched CMPs are CD34+CD38+CD123+CD45RA-, highly enriched GMPs are CD34+CD38+CD123+CD45RA+, and highly enriched MEPs are CD34+CD38+CD123-CD45RA-. (C) Nonsupervised hierarchical clustering (Euclidean distance) heatmap (78) of intensities for the peptides identified in HSCs, CMPs, GMPs, and MEPs (shades of red) isolated from five different donors (shades of blue). The peptide intensities are centered and scaled and depicted in color shades from red to blue. The missing peptide intensity values are shown in white. (D) Volcano plot of differential analysis of proteins. Comparison of HSCs to the average of the three other cell types. Abbreviations: HSPC, hematopoietic stem and progenitor cell; HSC, hematopoietic stem/multipotent progenitor cell; CMP, common myeloid progenitor; CLP/MLP, common/multipotent lymphoid progenitor; GMP, granulocyte-macrophage progenitor; MEP, megakaryocyte-erythrocyte progenitor; SSC, side scatter; FSC, forward scatter.